PCR-RFLP typing of ureC from Helicobacter pylori isolated from gastric biopsies during a European multi-country clinical trial

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens

Helicobacter pylori is the causative agent of chronic gastritis and peptic ulcer diseases and is also a risk factor for gastric cancer. Since culture of Helicobacter pylori is relatively insensitive and cumbersome, PCR-based molecular detection and typing of this organism is gaining importance for strain differentiation. The aim of present study was to apply molecular methods for detection and ...

pcr and rflp analysis for identification and typing of helicobacter pylori strains from gastric biopsy specimens

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Genotyping of the Helicobacter pylori cagA Gene Isolated From Gastric Biopsies in Shiraz, Southern Iran: A PCR-RFLP and Sequence Analysis Approach

BACKGROUND Cytotoxin-associated gene A (cagA) is an important virulence factor in the pathogenesis of Helicobacter pylori. OBJECTIVES The aim of this study was to genotype the H. pylori cagA gene isolated from antral biopsies of patients with stomach symptoms, using a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. PATIENTS AND METHODS A total of 161 gastric biopsies were ...

Histological Changes In Gastric Biopsies From Helicobacter Pylori-Infected Patients With And Without Peptic Ulcer

  Background and Objective: Long period risk of gastric carcinoma in people affected with chronic Helicobacter pylori gastritis is 5 times more than normal people. Perhaps histological changes due to helicobacter pylori infection could be used for screening people at risk of gastric carcinoma and peptic ulcer. Materials and Methods: This study has been carried out as a cross...